abstract：:Chromatographic discs were investigated for their potential to substitute for the hitherto used cartridges in heterogeneous flow injection analysis. Originally designed for fast high performance liquid chromatography (HPLC) of biopolymers, the discs combine reliability with speed and resolution. This together with the...

journal_title：Journal of biotechnology

authors： Hagedorn J,Kasper C,Freitag R,Tennikova T

更新日期：1999-03-26 00:00:00

abstract：:The construction of expression vectors encoding either the human insulin A- or B-chains fused to a synthetic peptide and the temperature-induced expression of the recombinant genes in Escherichia coli are reported. Using this two-chain approach we also describe the separate isolation of the insulin A- and B-chains fro...

journal_title：Journal of biotechnology

authors： Schmidt M,Babu KR,Khanna N,Marten S,Rinas U

更新日期：1999-02-05 00:00:00

abstract：:A fibrous-bed bioreactor containing the coculture of Pseudomonas putida and P. fluorescens immobilized in a fibrous matrix was developed to degrade benzene (B), toluene (T), ethylbenzene (E), and o-xylene (X) in synthetic waste streams. The kinetics of BTEX biodegradation by immobilized cells adapted in the fibrous-be...

journal_title：Journal of biotechnology

authors： Shim H,Yang ST

更新日期：1999-01-22 00:00:00

abstract：:E-lysis of Ralstonia eutropha H16, which belongs to the beta-subclass, was undertaken to verify whether transmembrane tunnel formation is possible in bacteria which do not belong to the enterobacteriaceae. For this purpose, a new gene E expression plasmid, pKG12, with two origins of replication, oriV and oriT, from pl...

journal_title：Journal of biotechnology

authors： Schroll G,Resch S,Gruber K,Wanner G,Lubitz W

更新日期：1998-12-11 00:00:00

abstract：:Catalytic properties and conformational stability of aminoacylase (N-acylamino acid amidohydrolase, EC 3.5.1.14) were studied in water-N,N-dimethylformamide (DMF) and water-dioxane solvent mixtures. Beside the prompt inhibition the solvents caused further inactivation during incubations. In the presence of 5% DMF cont...

journal_title：Journal of biotechnology

authors： Boross L,Kosáry J,Stefanovits-Bányai E,Sisak C,Szajáni B

更新日期：1998-11-18 00:00:00

abstract：:The poly(beta-hydroxybutyrate) (PHB) biosynthetic genes of Ralstonia eutropha that are organized in a single operon (phaCAB) have been cloned in Escherichia coli, where the expression of the genes in the wild-type pha operon from plasmid pTZ18U-PHB leads to the formation of 50-80% PHB/celldry mass when the cells are g...

journal_title：Journal of biotechnology

authors： Resch S,Gruber K,Wanner G,Slater S,Dennis D,Lubitz W

更新日期：1998-10-27 00:00:00

abstract：:The transfer of genetic information between distantly or even unrelated organisms during evolution had been inferred from nucleotide sequence comparisons. These studies provided circumstantial evidence that in rare cases genes had been laterally transmitted amongst organisms of the domains bacteria, archaea and eukary...

journal_title：Journal of biotechnology

authors： Dröge M,Pühler A,Selbitschka W

更新日期：1998-09-17 00:00:00

abstract：:Oxygen is a key substrate in animal cell metabolism. It has been reported that the oxygen uptake rate (OUR) is a good indicator of cellular activity, and even under some conditions, a good indicator of the number of viable cells. The measurement of OUR is difficult due to many different reasons. In particular, the ver...

journal_title：Journal of biotechnology

authors： Ruffieux PA,von Stockar U,Marison IW

更新日期：1998-08-12 00:00:00

abstract：:A D10A mutation was introduced at the 5'-3' exonuclease domain of Streptococcus pneumoniae DNA polymerase I by site directed mutagenesis of the polA gene. Introduction of the mutation resulted in a drastic decrease of the 5'-3' exonucleolytic activity present in the wild-type enzyme. Moreover, the mutation at the D10 ...

journal_title：Journal of biotechnology

authors： Amblar M,Sagner G,López P

更新日期：1998-07-30 00:00:00

abstract：:Several green fluorescent protein (Gfp) mutants with increased cellular fluorescence compared to the wildtype protein have recently been generated. We have expressed and compared wildtype Gfp and mutants S65T, F100S/ M154T/V164A, F64L/S65T, and S65A/V68L/S72A under identical growth conditions in CHO and Saccharomyces ...

journal_title：Journal of biotechnology

authors： Natarajan A,Subramanian S,Srienc F

更新日期：1998-06-11 00:00:00

abstract：:Poly(methyl methacrylate-acrylic acid) microspheres were prepared and the acid groups were activated by using either carbodiimide (CDI) or thionyl chloride (SOCl2). alpha-Amylase was covalently bound on these activated microspheres. The properties of the immobilized enzyme were investigated and compared with those of ...

journal_title：Journal of biotechnology

authors： Aksoy S,Tumturk H,Hasirci N

更新日期：1998-02-05 00:00:00

abstract：:A striking property of many prokaryotes is their enormous metabolic flexibility with respect not only to catabolic and anabolic substrates but also with respect to the continuously changing availability of nutrients. The phenotypic responses to low-nutrient growth conditions involve structural changes in the cellular ...

journal_title：Journal of biotechnology

authors： Teixeira de Mattos MJ,Neijssel OM

更新日期：1997-12-17 00:00:00

abstract：:Transformed cell cultures of Salvia miltiorrhiza were established by infecting sterile plantlets with Agrobacterium tumefaciens strain C58. The transformed cells in suspension formed macroscopic clumps of cell aggregates up to 2-3 cm in size rather than homogeneous cell suspensions. These transformed cells grew well i...

journal_title：Journal of biotechnology

authors： Chen H,Yuan JP,Chen F,Zhang YL,Song JY

更新日期：1997-12-03 00:00:00

abstract：:There are currently four crystal structures of low molecular weight endo-1,4-beta-xylanases (E.C.3.2.1.8), i.e. family G/11 xylanases, available at the Brookhaven Data Bank: 2 xylanases from Trichoderma reesei (Törrönen et al., 1994; Törrönen and Rouvinen, 1995) and one from Bacillus circulans and another from Trichod...

journal_title：Journal of biotechnology

authors： Törrönen A,Rouvinen J

更新日期：1997-09-16 00:00:00

abstract：:In this paper we describe the antigen recognition characteristics, variable region base and amino acid sequence, and performance as immunoaffinity chromatography ligands of two MAb specific to the alpha determinant of the HBsAg, derived from the same fusion. We show that the epitope recognized by CB-Hep.0 (IgM) is pro...

journal_title：Journal of biotechnology

authors： Fernández de Cossío ME,Díaz T,Galván A,Valdéz R,González E,Ayala M,Díaz J,Bestagno M,Burrone O,Gavilondo J

更新日期：1997-08-11 00:00:00

abstract：:A novel expression vector for yeast has been constructed from the regulatory elements present in the polI promoter and the enhancer/termination region (E/T) of rDNA. Under some conditions, this promoter/vector combination produces small RNAs such as the hammerhead RNA sequence at levels comparable to polII- and polIII...

journal_title：Journal of biotechnology

authors： Blancafort P,Ferbeyre G,Sariol C,Cedergren R

更新日期：1997-07-23 00:00:00

abstract：:Plasmid-free and plasmid-harbouring E. coli JM109 strains were investigated in shaken flasks, stirred tanks in batch and continuous operation. The shaken flask cultivations were performed in M9 minimal medium and in media with various protein supplements. The host hardly grows on M9 minimal medium as opposed to the pl...

journal_title：Journal of biotechnology

authors： Rhee JI,Bode J,Diaz-Ricci JC,Poock D,Weigel B,Kretzmer G,Schügler K

更新日期：1997-06-13 00:00:00

abstract：:Plasmid instability of a recombinant Saccharomyces cerevisiae C468/pGAC9 (ATCC 20690) was examined during continuous culture in a nonselective medium in an airlift bioreactor. The recombinant strain contained a 2-micron based shuttle vector pGAC9 and expresses Aspergillus awatnori glucoamylase gene under the control o...

journal_title：Journal of biotechnology

authors： Zhang Z,Scharer JM,Moo-Young M

更新日期：1997-05-23 00:00:00

abstract：:The use of high capacity micron-sized non-porous magnetic metal chelator adsorbents for the direct recovery of a recombinant metal-binding protein from crude liquors is described. Selectivity and interaction strength of magnetic chelator particles were assessed using a set of native proteins with known behaviour towar...

journal_title：Journal of biotechnology

authors： O'Brien SM,Sloane RP,Thomas OR,Dunnill P

更新日期：1997-04-04 00:00:00

abstract：:A control strategy to avoid development of synchronous growth in carbohydrate limited Saccharomyces cerevisiae cultivations is proposed and experimentally investigated. The basic idea is to control the metabolic flux through the pathways by manipulating the substrate feed rate to keep the ethanol concentration at a lo...

journal_title：Journal of biotechnology

authors： Møller H,Jørgensen SB

更新日期：1997-02-28 00:00:00

abstract：:In n-hexadecane fermentation by Pseudomonas aeruginosa, the production of rhamnolipids (biosurfactants) caused by nitrogen source limitation was observed during the stationary phase. The rhamnolipids caused severe foaming in the process, particularly at higher pH (ca. > 6.8). To reduce the foaming, several runs were m...

journal_title：Journal of biotechnology

authors： Wu J,Ju LK

更新日期：1997-01-03 00:00:00

abstract：:With the objective of monitoring xenobiotic degrading bacteria in soil, a method for rapid extraction of DNA from soil, amenable to amplification by PCR, was developed. The method was based on lysis by freeze-thawing and subsequent addition of sodium dodecyl sulfate (SDS), hexadecyltrimethylammonium bromide and protei...

journal_title：Journal of biotechnology

authors： Wikström P,Wiklund A,Andersson AC,Forsman M

更新日期：1996-12-10 00:00:00

abstract：:Xylanase T-6 is a thermostable alkaline-tolerant enzyme that is produced by Bacillus stearothermophilus T-6. Xylanase T-6 was found to bleach pulp effectively at pH 9 and 65 degrees C and was used successfully on an industrial-scale mill trial. To facilitate the future characterization of the protein via X-ray analysi...

journal_title：Journal of biotechnology

authors： Lapidot A,Mechaly A,Shoham Y

更新日期：1996-11-15 00:00:00

abstract：:An on-line model, estimating key state variables in bioprocesses, is utilized for control of fed-batch baker's yeast production. The state estimates are produced by balances and phenomenological expressions combined with on-line measurements. The goal of the control strategy is to maintain the highest possible glucose...

journal_title：Journal of biotechnology

authors： Ringbom K,Rothberg A,Saxén B

更新日期：1996-10-18 00:00:00

abstract：:A new and simple method was devised to obtain immunoglobulin VH genes directly from primary cultured B cells specific for a short peptide. Peptide-specific B cells were separated from splenocytes of peptide-immunized BALB/c mice with antigen-coated magnetic beads, and were cloned by a limitedly diluted culture in the ...

journal_title：Journal of biotechnology

authors： Takahashi Y,Ametani A,Totsuka M,Kaminogawa S

更新日期：1996-08-20 00:00:00

abstract：:The synthesis of 4-methylumbelliferyl 3-beta-O-cellobiosyl-beta-D-glucopyranoside (3a) and its use as specific substrate to monitor enzyme activity of 1,3-1,4-beta-D-glucan 4-glucanohydrolases are described. The chromophoric substrate 3a is prepared by a chemoenzymatic approach starting from barley grain, whose beta-D...

journal_title：Journal of biotechnology

authors： Malet C,Vallés J,Bou J,Planas A

更新日期：1996-07-31 00:00:00

abstract：:The use of a proper signal sequence is one of the major determinants for the efficient secretion of heterologous proteins from yeast. The signal sequence derived from inulinase (INU1A) of Kluyveromyces marxianus was evaluated in directing the secretion of a human glycoprotein, alpha 1-antitrypsin (alpha 1-AT), from Sa...

journal_title：Journal of biotechnology

authors： Kang HA,Nam SW,Kwon KS,Chung BH,Yu MH

更新日期：1996-07-18 00:00:00

abstract：:Escherichia coli phytase was covalently immobilized on NHS-activated Sepharose High Performance. The pH dependence of the phytase activity was not influenced by immobilization, whereas stability against heat treatment was enhanced as a consequence of immobilization. Compared to the free phytase the immobilized enzyme ...

journal_title：Journal of biotechnology

authors： Greiner R,Konietzny U

更新日期：1996-07-18 00:00:00

abstract：:NIZEMI (slow rotating centrifuge microscope) is a tool for optical investigations of small biological and non-biological specimens during variable accelerations. Two laboratory models for ground research designed for accelerations from 1 to 5 x g and 10 x g respectively are used for terrestrial research, especially i...

journal_title：Journal of biotechnology

authors： Friedrich UL,Joop O,Pütz C,Willich G

更新日期：1996-06-27 00:00:00

abstract：:The configuration and performance of the Biorack facility during the Second International Microgravity Laboratory mission (IML-2; 8-23 July 1995) is described in detail. During this Spacelab mission, Biorack flew with two incubators (22 degrees C and 37 degrees C), glovebox, cooler (5 degrees C) and four passive ther...

journal_title：Journal of biotechnology

authors： Manieri P,Brinckmann E,Brillouet C

更新日期：1996-06-27 00:00:00

abstract：:In this report, we describe the results of a rat pituitary cell culture experiment done on STS-65 in which the effect of cell feeding on the release of the six anterior pituitary hormones was studied. We found complex microgravity-related interactions between the frequency of cell feeding and the quantity and quality ...

journal_title：Journal of biotechnology

authors： Hymer WC,Grindeland RE,Salada T,Cenci R,Krishnan K,Mukai C,Nagaoka S

更新日期：1996-06-27 00:00:00

abstract：:This study evaluates the suitability of flow cytometry with the fluorochrome BCECF for measuring the intracellular pH (pHi) of cultured cells, and monitors the changes in pHi in murine hybridoma in batch culture and chick embryo fibroblast in monolayer culture (5th passage). The technique produced highly reproducible,...

journal_title：Journal of biotechnology

authors： Franck P,Petitipain N,Cherlet M,Dardennes M,Maachi F,Schutz B,Poisson L,Nabet P

更新日期：1996-05-15 00:00:00

abstract：:Enteric infections associated with bacteria, viruses and parasites are a world health problem accounting for at least one billion episodes of diarrhoea and up to 10 million deaths in the Third World each year. The widely held conclusion that responses which are protective in the gastrointestinal tract are best stimula...

journal_title：Journal of biotechnology

authors： Stokes CR,Bailey M

更新日期：1996-01-26 00:00:00

abstract：:Widely used inactivated vaccines for foot-and-mouth disease (FMD) induce protective immunity, but vaccine production plants and residual virus in the vaccine itself have been implicated in disease outbreaks. The structure of the FMD virion has been determined, and although much of the surface of the viral particle is ...

journal_title：Journal of biotechnology

authors： McKenna TS,Rieder E,Lubroth J,Burrage T,Baxt B,Mason PW

更新日期：1996-01-26 00:00:00

abstract：:Expression of cloned PhiX174 gene E in bacteria results in lysis of bacteria. It is unique among phage lysis systems as it introduces a transmembrane tunnel structure through the cell envelope complex of Gram-negative bacteria. The resulting bacterial ghosts have intact envelope structures devoid of cytoplasmic conten...

journal_title：Journal of biotechnology

authors： Szostak MP,Hensel A,Eko FO,Klein R,Auer T,Mader H,Haslberger A,Bunka S,Wanner G,Lubitz W

更新日期：1996-01-26 00:00:00

abstract：:The elemental composition of microorganisms varies widely with respect to the N, S, P and ash content as well as many trace elements depending on microorganism and growth conditions. As a consequence, the mass of 1 C-mol of biomass can differ considerably from one microorganism to another. Experimental results show th...

journal_title：Journal of biotechnology

authors： Duboc P,Schill N,Menoud L,van Gulik W,von Stockar U

更新日期：1995-12-01 00:00:00

abstract：:Survival of media-suspended porcine erythrocytes exposed to various hydrodynamic environments was investigated with and without such shear protectant additives as bovine serum albumin, dextran and the non-ionic surfactant Pluronic F68. Erythrocytes provided a model cell population with cells of a uniform size, metabol...

journal_title：Journal of biotechnology

authors： Zhang Z,Chisti Y,Moo-Young M

更新日期：1995-11-21 00:00:00

abstract：:Protein engineering has been employed to investigate the effect of specific amino acid changes on the targeting of heterologous proteins to the outer cell surface of the Gram-positive bacterium Staphylococcus xylosus. Three different variants, corresponding to a 101 amino acid region of the major glycoprotein (G prote...

journal_title：Journal of biotechnology

authors： Nguyen TN,Gourdon MH,Hansson M,Robert A,Samuelson P,Libon C,Andréoni C,Nygren PA,Binz H,Uhlén M

更新日期：1995-10-16 00:00:00

abstract：:Medium composition and cultivation conditions were constructed for the optimum production of leupeptin by Streptomyces exfoliatus SMF13. The production of leupeptin was related to mycelial growth, being optimum in the cultivation with glucose-excess, phosphate-limited, and casamino acids medium. However, leupeptin-ina...

journal_title：Journal of biotechnology

authors： Kim IS,Lee KJ

更新日期：1995-08-15 00:00:00

abstract：:This review is aimed at biochemists and molecular biologists, and covers the chemistry and key features involved in the solid-phase synthesis of a variety of the better known DNA and RNA analogues by the phosphoramidite and H-phosphonate methods. A wide spectrum of biological applications such as inhibition of gene ex...

journal_title：Journal of biotechnology

authors： Sproat BS

更新日期：1995-07-31 00:00:00